Providing a nanofibrillar matrix for in vivo-like cell culture performance

SurModics® Synthetic ECM is a synthetic, stable, and scalable new cell culture platform that provides an in vivo-like cell culture environment with its biomimetic extracellular matrix structure and surface. SurModics Synthetic ECM technology produces consistent, reproducible, and biologically meaningful results in an easy-to-use, cost-effective, and time-efficient manner for cell research and cell-related applications. The nanofibrillar matrix utilizes Ultra-WebTM technology.

Two format choices are currently available:

Nanofiber inserts, uncoated

Nanofiber inserts, polyamine surface

Nanofiber Inserts, Uncoated
These inserts provide a synthetic nanofibrillar electrospun cell culture surface that mimics the architecture of the basement membrane (BM), a specialized form of the extracellular matrix (ECM) within in vivo systems. This surface provides physiologically important nanotopography for cell attachment, growth, maintenance, and cell-to-cell interactions that more accurately reflect the in vivo environment.

Nanofiber Inserts, Polyamine Surface
These inserts provide a synthetic nanofibrillar electrospun cell culture surface that has been modified with a polyamine surface treatment. This modified surface provides nanotopography with enhanced surface biochemistry for cell attachment, growth, maintenance, and cell-to-cell interactions that more accurately reflect the in vivo environment. It may be used to covalently attach specific biomolecules for desired cellular functions.

ECM Product Information

Sterility
Product has been irradiated with electron beam processing

Stability
Product remains stable if stored at room temperature and out of direct sunlight. An accelerated stability test was performed with the accelerated challenge providing equivalency to 384 days at room temperature.

Quality Control
Each lot of SurModics Synthetic ECM is monitored for uniformity of fiber size distribution and tested in an attachment assay using the PC12 cell line.

Cells Cultured on SurModics Synthetic ECM
Please see Peer-Reviewed Publications on right for details on the following cells that were cultured on SurModics Synthetic ECM:
· NRK (normal rat kidney)
· NIH 3T3 (mouse fibroblast)
· T-47D (human breast epithelial)
· MCF7 (human breast adenocarcinoma)
· Mouse embryonic stem cell
· Primary rat cerebellar granule neuron
· Primary rat cerebral cortical neuron
· Primary rat hippocampal neuron
· Primary rat ventral spinal cord neuron
· Primary rat dorsal root ganglion neuron
· Primary rat astrocyte
· PC-12 (rat adrenal pheochromcytoma cell line) (Data not published)

ECM Product Partner

In May of 2006, SurModics entered into a strategic marketing and distribution agreement with Corning Incorporated, through which Corning Life Sciences will provide worldwide marketing and distribution of other nanofiber cell culture products for in vitro cell culture research and drug discovery applications. Corning Life Sciences currently offers 100 mm dishes and 96-well plates with Ultra-Web. For more information on these products, please visit Corning’s website.